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This book explores the latest advancements in rolling circle amplification (RCA) technology, focusing on its applications in clinical diagnostics and molecular medicine. It discusses new enzymes for RCA, techniques for enhanced signal amplification, innovative RCA diagnostics, and sensors to speed up RCA detection, as well as future RCA-based therapeutics. Targeted at university professors, students in biomedical engineering and biomolecular pharmacology, and R&D managers in biotechnology and biopharmaceutical sectors, it offers valuable insights. Key highlights include a review of RCA-derived DNA nanoparticles that effectively bind to cancer cells, an exploration of sensor systems using probe-tagged magnetic nanobeads for quicker detection of RCA products, and advancements in RCA diagnostics like PNA openers-assisted RCA for single target cell detection and in situ diagnosis of cancer cells. The book also presents innovative methods for enhancing RCA-generated signals, including nicking enzyme-assisted cascade RCA and RCA combined with loop-mediated amplification. Advance praise emphasizes the book's comprehensive overview of innovative RCA methods and applications, showcasing the evolution of the field and its potential for exciting developments in research and diagnostics.
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Rolling Circle Amplification (RCA), Vadim V. Demidov
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- Année de publication
- 2018
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