Strain and process engineering to exploit solvent tolerance mechanisms of Pseudomonas taiwanensis VLB120 for asymmetric styrene epoxidation

Auteurs

• Jan Volmer

Paramètres

• Manuels
• Manuels universitaires

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To mitigate the environmental impact of chemical production and reduce reliance on finite fossil resources, whole-cell biocatalysis emerges as a sustainable alternative. However, the apolar nature of target compounds often hinders biocatalyst efficiency due to toxic effects on enzymes and microorganisms. This research evaluates solvent-tolerant P. taiwanensis VLB120 as a biocatalyst to enhance the economic and ecological viability of producing asymmetric (S)-styrene oxide. It integrates biocatalyst development with reaction and process engineering, focusing on critical aspects and potential synergies from varying environments and design goals. A regulatory mutant, P. taiwanensis VLB120∆C∆ttgV, was engineered for constitutive solvent tolerance, eliminating the need for unproductive solvent-adaptation phases and doubling the specific styrene epoxidation activity of resting cells. Under glucose excess conditions, the highest specific styrene epoxidation rates (180 U gCDW-1) were achieved in a two-liquid phase biotransformation setup. The constitutive solvent tolerance also enabled a reduction in extractive organic phase volume by up to 90%, addressing significant environmental and economic concerns. Detailed analysis of glucose metabolism and solvent tolerance led to the identification of additional biocatalyst and process design objectives and engineering strategies.

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• Année de publication: 2016