Multiphoton microscopy and fluorescence lifetime imaging

Auteurs

• Karsten König

Paramètres

• Santé & Médecine

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This monograph delves into modern femtosecond laser microscopes for two-photon imaging and nanoprocessing, laser tweezers for cell micromanipulation, and fluorescence lifetime imaging (FLIM) in Life Sciences. It begins with an introduction by Dr. Wolfgang Kaiser, a pioneer in nonlinear optics, and concludes with a chapter on clinical multiphoton tomography, a cutting-edge high-resolution imaging technique. The foreword is provided by Dr. Colin Sheppard, an expert in nonlinear microscopy. The content is structured into three parts: Part I covers the basics, including a brief history of fluorescence lifetime imaging, the development of lasers for nonlinear optics, advanced TCSPC-FLIM techniques, and the role of ultrafast lasers in biophotonics. Part II focuses on modern nonlinear microscopy of live cells, discussing STED microscopy, temporal-focusing wide-field two-photon microscopy, FLIM-FRET microscopy, and the use of laser tweezers in two-photon effects. It also examines metabolic imaging and shifts in cell proliferation and differentiation, alongside femtosecond laser nanoprocessing and cryomultiphoton imaging. Part III addresses nonlinear tissue imaging, featuring Multiphoton Tomography (MPT), clinical multimodal CARS imaging, and in vivo multiphoton microscopy of human skin. It explores two-photon microscopy and FLIM of the cornea, multiscale imaging of the brain, and the interactions of dyes and nanomaterials. The se

Achat du livre

• Année de publication: 2018
• Reliure: (rigide)