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This book summarizes the biology of microRNA (miRNA) in various pathological processes, highlighting their potential in diagnostics, prognostics, and as novel drug targets. It covers traditional detection techniques such as standard PCR, Northern blotting, microarrays, and cloning methods. Additionally, it explores emerging strategies for miRNA detection and quantification, emphasizing new molecular biological techniques, locked nucleic acid (LNA) modified probes, and nanotechnology-based approaches. The latest advances in clinical miRNA detection methods in living cells, circulating blood, and tissues are discussed, including in situ hybridization (ISH) and molecular imaging techniques, which help elucidate miRNA biogenesis and function in vivo. The book also examines the advantages and drawbacks of various detection techniques in this rapidly evolving field, addressing challenges and promising new directions. It serves as a valuable resource for analytical chemists, biologists, and physicians engaged in miRNA research. The authors, Dr. Xueji Zhang and Dr. Haifeng Dong, are Professors at the School of Chemistry & Biological Engineering at the University of Science & Technology Beijing, while Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School.
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MicroRNA detection and pathological functions, Xueji Zhang
- Langue
- Année de publication
- 2015
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- (souple)
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