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This book addresses the gap created over the past five years in the study of recombinant ion channels, catering to both new and experienced researchers. It presents the latest techniques for ion channel research, contributed by professionals from major pharmaceutical companies who apply these methods in their work. The initial chapters focus on the Xenopus oocyte expression system for structure-function studies, covering basic to advanced approaches for manipulating ion channel cDNAs. Subsequent chapters review strategies for expressing channels in mammalian cells and analyzing them through patch-clamp electrophysiology. Later sections discuss modern methodologies for ion channel drug discovery, including high-throughput screening with fluorescence and luminescence, as well as automated planar array electrophysiology. The final chapters delve into determining ion channel crystal structures and computational methods for understanding channel mechanisms at atomic resolution. Rather than offering detailed protocols, the authors provide an accessible overview of techniques, discussing underlying principles, practical guidelines, and key considerations. Real-life examples from the authors' experiences and literature illustrate practical advice, making this a valuable resource for both academic and industrial professionals in the field.
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Expression and analysis of recombinant ion channels, Jeffrey J. Clare
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- Année de publication
- 2006
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