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This work-book provides step-by-step guidance through NMR experiments, starting with 1D routines and advancing to complex 3D experiments on proteins. It addresses key questions such as which experiment yields the desired information, how to perform it, how to interpret spectra, and the workings of specific pulse sequences. The third edition has been thoroughly revised and expanded to include 206 experiments across 15 chapters, covering a range of topics from routine spectra and variable temperature measurements to 1D multipulse experiments and solid-state materials. Notably, it introduces 3D experiments on the protein ubiquitin, consisting of 76 amino acids. This edition features 24 new experiments integrated into the existing chapters, including techniques like alpha/beta-SELINCOR-TOCSY and DOSY. Additionally, Chapter 15 introduces 20 new protein NMR experiments that require a special model sample of fully 13C- and 15N-labeled human ubiquitin, utilizing advanced techniques such as the constant time principle and gradient selection. Written by field experts, the guide emphasizes a hands-on approach, with all experiments performed as described and illustrated in the spectra. Serving as both a reference and a textbook, it is essential for scientists and students engaged in NMR laboratory work.
Achat du livre
200 and more NMR experiments, Stefan Berger
- Langue
- Année de publication
- 2004
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