Stavros Kromidas Livres

This practical guide for analytical scientists focuses on the use of gradients in liquid chromatography, which are essential for most chromatographic separations. It begins with the fundamentals of gradient separations and addresses common applications, including LC-MS coupling, biochromatography, and the separation of ionic substances. The book provides detailed, practical information to help practitioners use gradient separation methods effectively. It explores gradient optimization, covering special features, chromatographic definitions, and easy-to-remember rules for setting up gradient HPLC. Readers will find both linear and curvilinear models for predicting gradients, along with insights into the requirements, optimal use, and potential pitfalls of gradient elution. The guide discusses instrumental influences, gradient elution technology, and systematic characterization of gradient instrumentation. Additional topics include the use of gradients in LC-MS, specifications in biochromatography, separation of ionic components, hydrophilic interaction liquid chromatography (HILIC), and supercritical fluid chromatography (SFC). The book captures a decade of advancements in gradient HPLC, addressing everyday analytical challenges faced by novice and experienced users, and examining detection limits, peak capacity, resolution, and common misconceptions. It is designed for analytical chemists, chromatographers, environmental c

Filling the gap for an expert text dealing exclusively with the practical aspects of HPLC-MS coupling, this concise, compact, and clear book provides detailed information to enable users to employ the method most efficiently. Following an overview of the current state of HPLC-MS and its instrumentation, the text goes on to discuss all relevant aspects of method development. A chapter on tips and tricks is followed by user reports on the advantages - and pitfalls - of applying the method in real-life scenarios. The whole is rounded off by a look at future developments by renowned manufacturers.

How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful „HPLC Expert“ in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.

The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.

The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.

A unique approach to solving HPLC problems.Everyone who bought "Problem Solving in HPLC" by Stavros Kromidas will equally benefit from nearly 100 new practical examples for optimization, trouble-shooting, and instrument performance given in this sequel.The author provides- guidance for selecting and evaluating methods, intstruments and columns,- practical help with everyday trouble-shooting,- advice for optimizing separations, always explaining the reason why.In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in addition the book contains manufacturers' addresses, references, data tables and checklists.

Quick and easy solutions to HPLC problems! For 45 typical questions of HPLC users, the answer and general conclusions are presented on no more than 4 pages each. The book covers - simple equipment tests and selection criteria for columns, buffers etc. - specific problems and how to solve them - options for optimizing separations. The book also features a special chapter on the retention of ionizable components in RP-HPLC, references, data tables and check lists. It's a first-aid kit for every HPLC user. Stavros Kromidas is managing director of NOVIA, a company offering consulting services and training courses for analytical laboratories. For five years he was sales manager for the HPLC manufacturer Waters in Germany. He is author and editor of numerous books.

Erstmalig werden in einem Buch alle Aspekte der HPLC-Gradientenelution in den unterschiedlichen Trennmodi umfassend behandelt. Der Anwender findet konkrete Hinweise zur Optimierung von klassischen Lösungsmittelgradienten für RP- und LC-MS-Trennungen neben wertvollen Hinweisen zum Einsatz von Salz-, pH-Wert- und Temperaturgradienten - praxisnah und mit zahlreichen Beispielen. Teil 1 Aspekte der Gradienten-Optimierung in der RP-Chromatographie Apparative Einflüsse auf die Qualität von Gradienten-Methoden und deren Übertragung zwischen unterschiedlichen Geräten Optimierung einer Reversed Phase Gradiententrennung mit EXCEL Teil 2 Die Gradientelution ionischer Verbindungen Der Gradient in der Biochromatographie Spezifika der Gradientenelution in der HILIC Spezifika der Gradientenelution in der SFC Der Gradient in LC-MS-Messungen

Die „GC-Tipps“ ist ein kurzer, anwendungsorientierter Helfer für das GC-Labor. In bewusst leicht leserlicher Form werden Erklärungen, Ursachen-Auswirkungen und Konsequenzen vermittelt. Theoretische Betrachtungen werden äußerst dosiert dargeboten... Ein Praxisbuch von Praktikern für Praktiker. Teil 1 GC für Neueinsteiger – ein Leitfaden Teil 2 GC-Tipps (Auswahl) • Einstellung des Säulenvordrucks • Headspace – was ist das, was „bringt“ er? • Headspace Geräteparameter: Welche Einstellungen nehme ich am Gerät vor? • Welche GC - Injektionstechnik ist für meine Analytik die Richtige? • Probenvorbereitung in der GC – die Fallstricke • Leckagen in der GC-MS - wenn dem System die Luft ausgeht • Die Septen – einige Empfehlungen und Vorschläge • Welche Informationen liefert der FID? • Die Auswahl der stationären Phase • Das richtige Temperaturprogramm Teil 3 Anhang • „Geisterpeaks in der GC“ • Gaschromatographie heute – wo stehen wir?“