Harald Tschesche Livres

Frontmatter -- CONTENTS -- MODERN MICROMETHODS -- CHROMOGENIC PEPTIDE SUBSTRATES FOR THE DETERMINATION OF SERINE PROTEASES. A BRIEF REVIEW. -- IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY - A POWERFUL METHOD FOR PROTEIN PURIFICATION -- USE OF SYNTHETIC DYES FOR AFFINITY CHROMATOGRAPHY OF PROTEINS -- FLUORESCENCE-ACTIVATED CELL SORTING: ISOLATION OF GENETIC VARIANTS FOR THE ANALYSIS OF PROTEIN STRUCTURE AND FUNCTION -- THE FPLC*-SYSTEM FOR FAST AND HIGH RESOLUTION SEPARATIONS OF PROTEINS -- AMINO ACID ANALYSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND PRECOLUMN DERIVATISATION -- POTENTIALS OF HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY IN PROTEIN SEPARATIONS - -- SEPARATION OF CYANOGEN BROMIDE PEPTIDES OBTAINED FROM THE CATALYTIC DOMAIN OF THE NADPH-CYTOCHROME P-450 REDUCTASE (PIG LIVER) BY HPLC ON WIDE PORE COLUMN -- IMMUNOASSAYS OF PEPTIDES AND PROTEINS -- CHEMICAL MODIFICATION OF PROTEINS -- CROSS-LINKING REAGENTS -- MICRO-ANALYSIS OF POLYPEPTIDE STRUCTURE USING COLOR REAGENTS -- A MODULAR SYSTEM FOR MICROSEQUENCING OF PROTEINS, OLIGONUCLEOTIDE SYNTHESIS AND PEPTIDE SYNTHESIS -- ON-LINE DETECTION OF AMINO ACID DERIVATIVES RELEASED BY AUTOMATIC EDMAN DEGRADATION OF POLYPEPTIDES -- PROGRAMMABLE ELECTRONIC CONTROL OF SEQUENTIAL PROCEDURES OF ANALYTICAL DEVICES -- IMPROVED ISOCRATIC SEPARATION OF PHENYLTHIOHYDANTOIN AMINO ACIDS DOWN TO THE FEMTOMOLE LEVEL -- APPLICATION OF COMPUTER PROGRAMS FOR THE STRUCTURAL ANALYSIS OF PROTEINS -- DNA AND RNA SEQUENCE ANALYSIS -- SECONDARY STRUCTURE DETERMINATION OF MEMBRANE PROTEINS BY RAMAN SPECTROSCOPY -- STANDARD METHODS FOR THE CHEMICAL SYNTHESIS OF PEPTIDES -- AUTHOR INDEX -- SUBJECT INDEX

Frontmatter -- PREFACE -- CONTENTS -- STRATEGIES FOR THE LOCALIZATION AND SYNTHESIS OF PROTEIN BINDING SITES -- CONTINUOUS FLOW PEPTIDE SYNTHESIS -- SPECIFICITIES OF AUTOANTIBODIES TO SMALL NUCLEAR RIBONUCLEOPROTEINS (U snRNPs) IN SERA FROM PATIENTS WITH CONNECTIVE TISSUE DISEASES -- NEW ULTRASENSITIVE DETECTION SYSTEMS fOR BIOLUMINESCENCEENHANCED ENZYME IMMUNOASSAYS -- PRACTICAL CONSIDERATIONS IN BIOLUMINESCENCE-ENHANCED ENZYME IMMUNOASSAYS ILLUSTRATED BY A NEUTRAL PROTEINASE INHIBITOR OF PORCINE LEUKOCYTES -- A NEW, VERY SENSITIVE, BIOLUMINESCENCE-ENHANCED DETECTON SYSTEM fOR PROTEIN BLOTTING (WESTERN BLOT) -- THE USE OF COLLOIDAL METAL PARTICLES IN PROTEIN BLOTTING -- AMINO-ACID SEQUENCE ANALYSIS OF PROTEINS SEPARATED BY ONE- AND TWO-DIMENSIONAL GEL ELECTROPHORESIS AND ELECTROBLOTTED ON POLYBASE-COATED GLASSFIBER SHEETS -- PHOTOAFFINITY LABELING AND LOCALIZATION BY MICROSEQUENCING OF AN ION CHANNEL PROTEIN -- MICROCHARACTERIZATION OF PHOSPHOSERINE CONTAINING PROTEINSLOCALIZATION OF THE AUTOPHOSPHORYLATION SITES OF SKELETAL MUSCLE PHOSPORYLASE KINASE -- NEW MODULES FOR THE CONSTRUCTION OF A GASPHASE-SEQUENCER -- A NOVEL SOLID SUPPORT FOR GAS-LIQUID MICROSEQUENCING OF PROTEINS AND PEPTIDES - DERIVATIZED POROUS GLASS REPLACES POLYBRENE-COATED GLASFIBER FILTER -- NEW AUTOMATED AMINO ACID ANALYSIS BY HPLC PRECOLUMNS DERIVATISATION WITH FLUORENYLMETHYLOXYCARBONYLCHLORIDE -- HICROCALORIMETRY OF PROTEIN - LIGAND INTERACTION AND PROTEIN UNFOLDING -- APPLICATION OF BIFUNCTIONAL REAGENTS FOR TOPOLOGICAL INVESTIGATION -- TOWARDS THE IDENTIFICATION OF THE NUCLEOTIDE-BINDING SITE OF TUBULIN -- CRYSTALLOGRAPHIC AND IMAGE RECONSTRUCTION STUDIES ON RIBOSOMES -- Cell Culture Techniques for Testing of Biologically Active Peptides and Drugs: Clonogenic Assays Using Agar-Containing Glass Capillaries -- ELECTROTRANSFORMATION OF CULTURE CELLS -- AUTHOR INDEX -- Subject Index

This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.