Emmanuel G. Reynaud Livres

An indispensable guide to a novel, revolutionary fluorescence microscopy technique! Light sheet-based fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a “do-it-yourself” light sheet microscope, making the technique affordable for every laboratory. This important Aimed at cell biologists, biophysicists, developmental biologists, and neurobiologists, Light Sheet-based Fluorescence Microscopy offers a comprehensive overview of the and the most recent applications of this microscopy technique.

Written by an international team of experts from the Tara Oceans Marine Biology Imaging Platform (TAOMI), this is the first and only compendium on marine imaging technologies, and includes all known underwater as well as on-land techniques. TAOMI is imaging the largest collection of marine organisms in recent history, ranging from viruses to corals, and is duplicated on land to perform high throughput confocal analysis of plankton, X-ray tomography as well as cryo-electron microscopy. This unique platform combines underwater imaging with cytometry, stereomicroscopy, fluorescence microscopy and 3D microscopy - all of which are covered in this practical book, along with remote sensing, MRI, and optical projection tomography. The definitive resource for every marine biologist who is planning to image marine species, whether underwater or on land.