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The research focuses on the interaction between HIV-1 TAR RNA and the viral protein Tat, crucial for viral genome transcription. By synthesizing RNA ligands using heteroaromatic building blocks, the study aims to develop specific antiviral Tat antagonists. Binding affinities were assessed through fluorescence assays and spectroscopy, revealing conformational changes in TAR RNA via NMR and molecular dynamics. Additionally, the antiviral effects of peptides and small molecules were evaluated using HeLa P4 cells, with a particular emphasis on diaminopyrazoles and indazoles.
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Peptidomimetic building blocks, Sven Breitung
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- Année de publication
- 2020
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